ELISA (Enzyme Linked Immunosorbent Assay)

ELISA has been used globally for the detection of various antibodies and antigens. This procedure is very simple and also as sensitive as radioimmunoassay. In ELISA, only a small quantity of test reagent is required and in this, the enzyme is used instead of fluorescent dye. ELISA can be used for the detection of antigen and antibody.

ELISA test

Uses of ELISA

ELISA is used for detection of a variety of antigens and antibodies of various microorganisms. Some of the examples are

  • Detection of HIV antibodies in serum
  • Detection of mycobacterial antibodies in tuberculosis
  • Detection of rotavirus in faeces
  • Detection of hepatitis B markers in serum
  • Detection of enterotoxin of Escherichia Coli in faeces

What does ELISA Stand For

Elisa stands for Enzyme-Linked Immunosorbent Assay. ELISA is of three types

  • Sandwich ELISA
  • Indirect ELISA
  • Competitive ELISA

Dr Deepak Kansal is a dentist, who is currently working in Sri Ganganagar. He did his Bachelor of Dental Surgery from Rajasthan University of Health Science.

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